승마추출액을 처리한 G361세포에서의 apoptosis 효과에 대한 연구
Effect of apoptosis on G361 cells by Cimicifuga rhizoma extract
최별보라, 김규천, 홍진우, 박상례,
소속 상세정보
최별보라 ( Choi Byul-Bo-Ra ) - (주)피글
김규천 ( Kim Gyoo-Cheon ) - 부산대학교 치의학전문대학원 구강해부학교실
홍진우 ( Hong Jin-Woo ) - 부산대학교 한의학전문대학원 한방내과학교실
박상례 ( Park Sang-Rye ) - 경남정보대학교 치위생과
Abstract
Objectives: To investigate whether the cytotoxic effect of Cimicifuga rhizoma extract is associated with cell death in the human keratinocyte (HaCaT) and human melanoma cell lines (G361).
Methods: Apoptosis induced by Cimicifuga rhizoma extract was confirmed by water-soluble tetrazolium salts-1 (WST-1) assay, immunocytochemistry, and western blot. Additionally, the release of cytochrome c and apoptosis-inducing factor (AIF) was visualized by confocal laser scanning microscopy.
Results: The results showed that Cimicifuga rhizoma extract significantly reduced the viability of G361 cells with half-maximal inhibitory concentration (IC 50) of 200 μg/ml, and the apoptotic process was found to occur via the activation of caspase-3 and caspase-9 pathways. Besides, the release of cytochrome c and AIF was also detected.
Conclusions: This study suggests that Cimicifuga rhizoma extract causes apoptosis of human melanoma cells through the intrinsic apoptotic pathway.
키워드
Apoptosis; Cimicifuga rhizoma; Melanoma
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